F A C U L T Y   P R O F I L E 

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Boyden, Penelope A. Ph.D.
Professor Emeritus of Pharmacology

Cardiac electrophysiology; mechanisms of arrhythmias in experimental and naturally occurring animal models of disease heart.

Office: Physicians & Surgeons| 1st floor | Room 416A

Current Research

Cardiac electrophysiology; mechanisms of arrhythmias in experimental and naturally occurring animal models of disease. Research in our laboratory is dedicated to determining the electrophysiological basis of abnormal heart rhythms (cardiac arrhythmias). In one set of projects we enzymatically isolate single cells from hearts that have become arrhythmic due to experimental myocardial infarction, or pacing induced atrial fibrillation. We examine the function of specific ionic currents, in particular the sodium and potassium currents in the diseased cells and how this might be altered by drugs. Recently we are examining how Na channels are replenished on the surface of cells that survive in the infarcted heart and form the substrate for reentrant arrhythmias.

In other groups of experiments we use single Purkinje cells from diseased hearts in order to determine how intracellular calcium homeostasis is altered in cells of hearts post myocardial infarction. In some studies we combine whole cell voltage clamp techniques with epifluorescent and Ca2+ imaging techniques. We have found examples of reverse excitation contraction coupling where cytosolic Ca2+ elicits nondriven electrical activity.

Selected Publications

1. Dun W, Danilo P Jr, Curran J, Mohler PJ, Boyden PA. 2017. Microtubule Remodeling and Decreased Nav1.5 Expression in Myocytes from Epicardial Border Zone of the Infarcted Canine Heart (in press).

2. Reher T, Wang Z, Hseuh C_H, Chang PC, Pan Z, Kumar M, Patel J, Tan J, Shen C, Chen Z, Fishbein MC, Rubart M, Boyden PA, Chen P-S. 2017. Small conductance calcium activated potassium current in Normal rabbit Purkinje Cells. J Am Heart Assoc 26(6).

3. Boyden PA. Dun W, Robinson RB. 2016. Cardiac Purkinje fibers and Arrhythmias; The Gordon K Moe Award Lecture. Heart Rhythm.13(5):1172-81.

4. Boyden PA, Dun W Stuyvers B. 2015. What is a Ca2+ wave? Is it like an Electrical Wave? Arrhythmia and Electrophysiology Reviews, Arrhythm Electrophysiol Rev. May;4(1):35-9.

5. Dun W, Wright P, Danilo Peter Jr, Mohler PJ, Boyden PA. 2014. SAP 97 and Cortactin Remodeling in Arrhythmogenic Purkinje Cells. PLoS One. 9:e2014.

6. Dun W, Lowe JS, Wright P, Hund TJ, Mohler PJ, Boyden PA. 2013. Ankyrin-G participates in INa remodeling in myocytes from the border zone of the infarcted heart. PLOS ONE. 14;8(10):e78087

7. Liu N, Denegri M, Dun W, Protasi F, Boncompagni S, Protasi F, Volpe P, Napolitano C ,Boyden PA, Priori SG: 2013. Abnormal propagation of calcium waves and ultrastructural remodeling in recessive CPVT. Circ Res 5;113(2):142-52.

8. Gudmundsson H, Hund TJ, Wright PJ, Kline CF, Snyder JS, Qian L, Koval OM, Cunha SR, George M, Rainey MA, Kashef FE, Dun W, Boyden PA, Anderson ME, Band H, Mohler PJ. 2010. EH Domain Proteins Regulate Cardiac Membrane Protein Targeting. Circ Res;107(1):84-95.

9. Pallante BA, Giovannone S, Fang-Yu L, Zhang J, Liu N, Kang G, Dun W, Boyden PA, Fishman GI. 2010. Contactin-2 expression in the cardiac Purkinje fiber network.Circ Arrhythm Electrophysiol. 1;3(2):186-94.

10. Hirose M, Stuyvers B, Dun W, ter Keurs HEDJ and PA Boyden. 2008. Function of Ca2+ release Channels in Purkinje cells that survive in the Infarcted Canine Heart; a Mechanism for triggered Ventricular beats. Circ: Arrhythmia and Electrophysiology 1: 387-395

11. Baba S, Dun W, Cabo C, Boyden PA.2005. Remodeling in cells from different regions of the reentrant circuit during ventricular tachycardia. Circulation 112:2386-2396

12. Stuyvers BD, Dun W, Matkovich S, Sorrentino V, Boyden PA, ter Keurs HEDJ.2005. Confocal Analysis of Ca2+ Sparks and Waves in Canine Purkinje Cells; A Triple Layered System of Activation. Circulation Research 97:35-43